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Immunologie und Infektion

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实时测量中性粒细胞的线粒体生物能量特征

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Immunologie und Infektion

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JoVE Journal Immunologie und Infektion

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

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实时测量中性粒细胞的线粒体生物能量特征

DOI:

10.3791/64971-v

•

09:39 min

•

June 02, 2023

•

Sunitha Pulikkot, Meng Zhao³, Zhichao Fan

¹Department of Immunology, School of Medicine,UConn Health, ²Arthritis and Clinical Immunology Program,Oklahoma Medical Research Foundation, ³Department of Microbiology and Immunology,University of Oklahoma Health Science Center

Kapitel

00:05Introduction
01:05Preparation of the 96-Well Plate for the Metabolic Extracellular Flux Assay
01:52Preparation and Seeding of Cells
03:52Preparing Compounds in the Mitochondrial Stress Test Kit
04:25Running the Mitochondrial Stress Assay
07:29Results: Metabolic Flux Assay to Analyze Oxygen Consumption Rates (OCR) in Neutrophils
09:02Conclusion

Summary

Automatische Übersetzung

我们描述了使用代谢细胞外通量分析仪测量小鼠和人中性粒细胞以及HL60细胞线粒体呼吸的逐步方案。

Tags

Mitochondrial Respiration Neutrophils HL60 Cells Metabolic Extracellular Flux Analyzer Mitochondrial Function Cell Seeding Density Assay Medium Mouse Neutrophils Human Neutrophils Differentiated HL60 Cells Undifferentiated HL60 Cells Background Correction

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