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Xenograft Skin Model to Manipulate Human Immune Responses In Vivo
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Xenograft Skin Model to Manipulate Human Immune Responses In Vivo
Kapitel
- 00:04Introduction
- 00:59Processing of Donor Human Skin Sample
- 02:15Pre-Surgery Conditioning and Preparation
- 03:42Transplantation Procedure
- 06:09Results: Analysis of the Xenografts
- 07:35Conclusion
The present protocol describes how to graft human skin onto non-obese diabetic (NOD)-scid interleukin-2 gamma chain receptor (NSG) mice. A detailed description of the preparation of human skin for transplant, preparation of mice for transplant, transplantation of split-thickness human skin, and post-transplantation recovery procedure are included in the report.
