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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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JoVE Journal Chemie
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

DOI:
10.3791/63451-v

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08:40 min

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January 20, 2022

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, , ,
1INRAE, UR BIA, F-44316 Nantes, France, 2INRAE, BIBS Facility, F-44316 Nantes, France

Kapitel

  • 00:04Introduction
  • 01:15Tuning of the Cyclic IMS Mass Spectrometer
  • 02:39IMS/IMS-MS Acquisition
  • 06:08Results: Representative Results for the Cyclic IMS
  • 07:51Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Ion mobility spectrometry (IMS) is an interesting complement to mass spectrometry for the characterization of biomolecules, notably because it is sensitive to isomerism. This protocol describes a tandem IMS (IMS/IMS) experiment, which allows the isolation of a molecule and the generation of the mobility profiles of its fragments.

Tags

Cyclic Ion Mobility SpectrometerTandem Ion MobilityGlycan ClassificationMolecular NetworksMass SpectrometryIon MobilityArrival TimeCalibrationAcquisition SettingsTOF ModeMSMS Mode

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