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使用循环离子淌度光谱仪进行串联离子淌度实验

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Chemie

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JoVE Journal Chemie

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

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使用循环离子淌度光谱仪进行串联离子淌度实验

3,783 Views

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08:40 min

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January 20, 2022

DOI:

10.3791/63451-v

DOI:

10.3791/63451-v

•

08:40 min

January 20, 2022

•

22 Views

Simon Ollivier^1,2, Mathieu Fanuel^1,2, Hélène Rogniaux^1,2, David Ropartz^1,2

¹INRAE, UR BIA, F-44316 Nantes, France, ²INRAE, BIBS Facility, F-44316 Nantes, France

Summary

离子淌度光谱法（IMS）是质谱法表征生物分子的有趣补充，特别是因为它对异构体敏感。该协议描述了串联IMS（IMS / IMS）实验，该实验允许分离分子并生成其片段的迁移率曲线。

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