Journal
/
Immunologie und Infektion
/
Isolation of Double Negative αβ T Cells from the Kidney
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Isolation of Double Negative αβ T Cells from the Kidney

Isolation of Double Negative αβ T Cells from the Kidney

11,990 Views

•

06:56 min

•

May 16, 2014

DOI:

10.3791/51192-v

DOI:
10.3791/51192-v

•

06:56 min
May 16, 2014

•

3 Views
, , ,
1Department of Pathology,Johns Hopkins University School of Medicine, 2Department of Medicine,Johns Hopkins University School of Medicine

Summary

Automatically generated

DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine kidney.

Explore More Videos

Double Negative T CellsDN T CellsKidneyIsolation ProtocolCD45+ SelectionNegative SelectionCD4CD8MHC Class IICD1d α-galcer TetramerPurity

Read Article

Cite this Article

Martina, M. N., Bandapalle, S., Rabb, H., Hamad, A. R. Isolation of Double Negative αβ T Cells from the Kidney. J. Vis. Exp. (87), e51192, doi:10.3791/51192 (2014).

Download .ris file

Copy

Share Video

.

Copy