/
/
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
6,228 Views
•
05:37 min
•
October 20, 2020
DOI:
Summary
Automatically generated
Here, we present a protocol to profile the interplay between host and pathogen during infection by mass spectrometry-based proteomics. This protocol uses label-free quantification to measure changes in protein abundance of both host (e.g., macrophages) and pathogen (e.g., Cryptococcus neoformans) in a single experiment.
Read Article
Cite this Article
Ball, B., Sukumaran, A., Geddes-McAlister, J. Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions. J. Vis. Exp. (164), e61881, doi:10.3791/61881 (2020).
Copy