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Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

DOI:
10.3791/61034-v

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10:14 min

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February 27, 2021

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, ,
1Departamento de Biologia,Faculdade de Ciências da Universidade do Porto, 2Laboratório Associado para a Química Verde, 3Dipartimento di Bioscienze,Universitá degli Studi di Milano

Kapitel

  • 00:09Introduction
  • 00:39Sample Fixation and Dehydration
  • 02:17LR-White Resin Embedding
  • 03:26Sample Trimming and Sectioning
  • 05:12Immunolocalization
  • 07:31Results: Subcellular Localization of Proteins and Pectins
  • 09:32Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol describes in detail how the plant material for immunolocalization of Arabinogalactan proteins and pectins is fixed, embedded in a hydrophilic acrylic resin, sectioned and mounted on glass slides. We show cell wall related epitopes will be detected with specific antibodies.

Tags

Fluorescent ImmunolocalizationArabinogalactan ProteinsPectinsCell WallFixationVacuumDehydrationEmbeddingLR-white Resin

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