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单个细胞分辨率三维成像的有机体

JoVE Journal
Biologie

This content is Free Access.

JoVE Journal Biologie

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

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单个细胞分辨率三维成像的有机体

14,861 Views

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10:40 min

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June 05, 2020

DOI:

10.3791/60709-v

DOI:

10.3791/60709-v

•

10:40 min

June 05, 2020

•

44 Views

Ravian L. van Ineveld*^1,2,3, Hendrikus C.R. Ariese*^1,2,3, Ellen J. Wehrens^1,2,3, Johanna F. Dekkers*^1,2,3,4, Anne C. Rios*^1,2,3

¹Princess Máxima Center for Pediatric Oncology, ²Department of Cancer Research, Oncode Institute,Hubrecht Institute-KNAW Utrecht, ³Cancer Genomics Center (CGC), ⁴Hubrecht Institute,Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht

Summary

使用此处描述的单细胞分辨率 3D 成像方案，可以捕获器官的整个 3D 结构和细胞含量，以及它们与原始组织的表型相似性。该协议可应用于各种器官，其来源、大小和形状各不相同。

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