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Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay
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JoVE Journal Neurowissenschaften
Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay

Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay

DOI:
10.3791/59558-v

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08:33 min

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June 25, 2019

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, , ,
1Laboratory for Biomedical Neurosciences,Neurocenter of Southern Switzerland, 2Neurology Department,Neurocenter of Southern Switzerland, 3Department of Neurology, Inselspital, Bern University Hospital,University of Bern, 4Faculty of Biomedical Sciences,Università della Svizzera Italiana

Kapitel

  • 00:04Titel
  • 01:17Tissue Fixation and Storage
  • 02:15Tissue Cut with a Cryotome
  • 03:11Immunofluorescence Staining
  • 05:34Immunofluorescence Imaging
  • 06:01Results: Analysis of the Free-floating Immunofluorescence Assay
  • 08:09Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we present a protocol for a free-floating indirect immunofluorescence assay on skin biopsy sections that allows for the identification of disease specific conformation variants of alpha synuclein involved in Parkinson disease and multiple proteins of the peripheral nervous system.

Tags

Parkinson’s DiseaseSkin BiopsyAlpha Synuclein AggregatesPeripheral Nerve FibersImmunofluorescence AssayCryoprotectantCryosectioningBiomarkerPathogenesis

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