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Developmental Biology

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JoVE Journal Developmental Biology
Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
 

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Article DOI: 10.3791/57921-v • 09:07 min • June 10th, 2018
June 10th, 2018 •
*1, *1, 1, 1
1Department of Oncology, Division of Pediatric Oncology and Institute for Cell Engineering, Johns Hopkins School of Medicine
* These authors contributed equally

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Summary
Automatic Translation

We present a protocol for efficient, bulk, and rapid chemical reversion of conventional lineage-primed human pluripotent stem cells (hPSC) into an epigenomically-stable naïve preimplantation epiblast-like pluripotent state. This method results in decreased lineage-primed gene expression and marked improvement in directed multilineage differentiation across a broad repertoire of conventional hPSC lines.

Tags

Chemical Reversion Conventional Human Pluripotent Stem Cells Naive-like State Multilineage Differentiation Potency LIF-3i Method Biochemical Features Transcriptional Features Epigenetic Features Pre-implantation Epiblast Functionality Improvement Lineage-primed Gene Expression Interline Variability Directed Differentiation Karyotypic Stability Epigenomic Stability Imprinted Loci Improved Efficiencies Re-priming Step Early Human Developmental Biology Regenerative Medicine Gene Targeting Interspecies Chimeras Protocol
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