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Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

1,209 Views

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10:52 min

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March 22, 2024

DOI:

10.3791/66527-v

DOI:
10.3791/66527-v

•

10:52 min
March 22, 2024

•

68 Views
, , , ,
1Department of Bioengineering, Department of Medicine,University of Washington, 2Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

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This protocol presents the differentiation of human osteoclasts from induced pluripotent stem cells (iPSCs) and describes methods for the characterization of osteoclasts and osteoclast precursors.

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OsteoclastsHuman Induced Pluripotent Stem Cells (iPSCs)DifferentiationCharacterizationPassagingCD34+ Peripheral Blood Mononuclear CellsBone Disease ResearchKrebsforschungEndoprosthesis ResearchExpandabilityDMEM/F-12DispaseHEPESCell CultureCell Aggregates

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Blümke, A., Simon, J., Leber, E., Scatena, M., Giachelli, C. M. Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (205), e66527, doi:10.3791/66527 (2024).

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