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石蜡嵌段细胞免疫细胞化学法测定培养体细胞蛋白质表达水平
JoVE Journal
Krebsforschung
This content is Free Access.
JoVE Journal Krebsforschung
Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks
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石蜡嵌段细胞免疫细胞化学法测定培养体细胞蛋白质表达水平

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09:06 min

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May 20, 2018

DOI:

10.3791/57369-v

DOI:
10.3791/57369-v

•

09:06 min
May 20, 2018

•

7 Views
, , , ,
1Omics Core Lab, Research Institute,National Cancer Center, 2Department of Pathology,Inje University Ilsan Paik Hospital

Summary

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目前, 固定细胞免疫荧光染色是确定形态学信息时蛋白质表达水平的选择方法。本协议提供了一种替代方法的免疫细胞化学在石蜡嵌入单元块。

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Poojan, S., Kim, H., Yoon, J., Sim, H. W., Hong, K. Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks. J. Vis. Exp. (135), e57369, doi:10.3791/57369 (2018).

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