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通过显微注射卵母细胞生成转基因小鼠
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Generation of Genetically Modified Mice through the Microinjection of Oocytes
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通过显微注射卵母细胞生成转基因小鼠

20,031 Views

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10:19 min

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June 15, 2017

DOI:

10.3791/55765-v

DOI:
10.3791/55765-v

•

10:19 min
June 15, 2017

•

32 Views
,
1Transgenic Animal Unit, Mark Wainwright Analytical Centre,University of New South Wales

Summary

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小鼠卵母细胞的显微注射通常用于经典转基因（ 即转基因的随机整合）和CRISPR介导的基因靶向。该协议审查显微注射的最新发展，特别强调质量控制和基因分型策略。

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Cite this Article

Delerue, F., Ittner, L. M. Generation of Genetically Modified Mice through the Microinjection of Oocytes. J. Vis. Exp. (124), e55765, doi:10.3791/55765 (2017).

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