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엔테로 바이러스 및 문화 RT-qPCR에 의해 물에서 노로 바이러스의 발생의 EPA 방법 1615 측정. II. 총 배양 가능한 바이러스 분석
JoVE Journal
Umwelt
This content is Free Access.
JoVE Journal Umwelt
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
DOI:

11:09 min

September 11, 2016

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Kapitel

  • 00:05Titel
  • 02:32Filter Elution Procedure
  • 03:33Organic Flocculation Concentration Procedure
  • 06:03Total Culturable Virus Quantal Assay
  • 09:26Results: Mean Poliovirus Recovery (%) from Ground and Reagent-grade Water
  • 10:19Conclusion

Summary

Automatische Übersetzung

Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.

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