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JoVE Journal Developmental Biology
Optimering af renal organoid og organotypic kultur for vaskularisering, udvidet udvikling, og forbedret mikroskopi Imaging
 
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Optimering af renal organoid og organotypic kultur for vaskularisering, udvidet udvikling, og forbedret mikroskopi Imaging

Article DOI: 10.3791/60995-v • 12:49 min • March 28th, 2020
March 28th, 2020 •
1, 1, 1, 1, *1, *1,2,3
1Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, 2InfoTech Oulu, University of Oulu, 3Biobank Borealis of Northern Finland, Oulu University Hospital
* These authors contributed equally

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Dette arbejde beskriver to metoder til at studere organudvikling, en forbedret xenotransplantation setup på chorioallantoic membran (CAM) fra aviær embryoner, der giver mulighed for vaskularisering af dyrkede embryonale organer og organoider og en ny fast z-retning organkulturmetode med modificerede eksperimentelle betingelser, der giver mulighed for konfokal billeddannelse med høj opløsning.

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Udviklingsbiologi nyre organkultur time-lapse organoid imaging chick CAM kylling embryo chick ex ovo kultur vaskularisering xenotransplantation
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