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发育生物学
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Levende celle billeddannelse af kromosom Segregation under mitosen
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发育生物学
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JoVE 杂志 发育生物学
Live Cell Imaging of Chromosome Segregation During Mitosis

Levende celle billeddannelse af kromosom Segregation under mitosen

DOI:
10.3791/57389-v

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06:39 min

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March 14, 2018

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, ,
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research,US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research,US Food and Drug Administration

Chapters

  • 00:04Title
  • 01:04Labeling Cells
  • 02:21Visualization of Lagging Chromosomes
  • 04:45Results: Nocodazole-treated MEFs Lacking PP2A-B56 Undergo Chromosomal Mis-segregation
  • 05:29Conclusion

Summary

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自动翻译

Denne protokol beskriver en nem og bekvem metode til at mærke og visualisere live kromosomer i mitotiske celler ved hjælp af Histone2B-NGL BacMam 2.0 etiket og et roterende disksystem Konfokal mikroskopi.

Tags

Live Cell ImagingChromosome SegregationMitosisHistone 2B-GFP BacMam 2.0Spinning Disc Confocal MicroscopyMouse Embryonic FibroblastsNocodazoleCell SynchronizationLive Chromosome VisualizationEnvironmental ChamberOil Immersion Objective

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