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JoVE Journal Developmental Biology
Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging
 

Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging

Article DOI: 10.3791/60995-v • 12:49 min • March 28th, 2020
March 28th, 2020 •
1, 1, 1, 1, *1, *1,2,3
1Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, 2InfoTech Oulu, University of Oulu, 3Biobank Borealis of Northern Finland, Oulu University Hospital
* These authors contributed equally

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This work describes two methods for studying organ development, an improved xenotransplantation setup on chorioallantoic membrane (CAM) from avian embryos that allows for vascularization of cultured embryonic organs and organoids and a novel fixed z-direction organ culture method with modified experimental conditions that allows for high-resolution time-lapse confocal imaging.

Tags

Renal Organoid Organotypic Culture Vascularization Extended Development Microscopy Imaging Kidney Development Kidney Disease Organ Culture System Blood Flow Kidney Function Filtration Cultivation Visualization Embryo Kidneys Chorioallantoic Membrane Chicken Embryo Kidney Rudiments Permeable Meaning Reservoirs Culture Medium Endothelial Cells Glomerulus Fixed Set Direction Organ Culture Method High Resolution Confocal 3D Time-lapse Imaging Tissue Compression Glass Cover Slip Permeable Membrane
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