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从人类和小鼠心肌标本中分离巨噬细胞子集和细胞位细胞

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JoVE 杂志免疫与感染

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

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从人类和小鼠心肌标本中分离巨噬细胞子集和细胞位细胞

DOI:

10.3791/60015-v

•

07:25 min

•

December 17, 2019

•

Geetika Bajpai, Kory J. Lavine^2,3

¹Department of Medicine,Washington University School of Medicine, ²Department of Immunology and Pathology,Washington University School of Medicine, ³Department of Developmental Biology,Washington University School of Medicine

Chapters

00:05Title
01:14Preparation of Human Cardiac Tissue Specimens
01:41Preparation of the Mouse Heart
02:11Preparation of Single Cell Suspension
04:50Antibody Staining
05:33Results: Flow Cytometry Gating Scheme
06:22Conclusion

Summary

自动翻译

这里介绍的一个协议，通过酶消化制备单个细胞悬浮液，从人类和小鼠心肌中分离出巨噬细胞和其他非免疫细胞的各种子集。提出了基于流细胞学的识别与分离巨噬细胞表征的门控方案。

Tags

Macrophage Subsets Stromal Cells Cardiac Inflammation Cardiac Repair Immune Cell Diversity Single Cell Suspension Viable Immune Cells Nonimmune Cell Populations Murine Heart Collagenase I DNase I Hyaluronidase DMEM Digestion

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