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小鼠视网膜冷冻节的制备,用于免疫性植物化学

JoVE 杂志
神经科学

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JoVE 杂志神经科学

Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

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小鼠视网膜冷冻节的制备,用于免疫性植物化学

25,080 Views

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05:25 min

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July 01, 2019

DOI:

10.3791/59683-v

DOI:

10.3791/59683-v

•

05:25 min

July 01, 2019

•

63 Views

Hélène Léger¹, Evelyn Santana², William A Beltran², Francis C Luca¹

¹Department of Biomedical Sciences,University of Pennsylvania School of Veterinary Medicine, ²Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine,University of Pennsylvania School of Veterinary Medicine

Summary

本报告介绍了制备冷冻小鼠视网膜部分用于免疫组织化学(IHC)的综合方法。所述方法包括眼部后杯的解剖、甲醛固定、嵌入最佳切割温度(OCT)介质和组织定向、切片和免疫染色。

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