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JoVE Journal Developmental Biology
Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
 

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Article DOI: 10.3791/57921-v • 09:07 min • June 10th, 2018
June 10th, 2018 •
*1, *1, 1, 1
1Department of Oncology, Division of Pediatric Oncology and Institute for Cell Engineering, Johns Hopkins School of Medicine
* These authors contributed equally

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We present a protocol for efficient, bulk, and rapid chemical reversion of conventional lineage-primed human pluripotent stem cells (hPSC) into an epigenomically-stable naïve preimplantation epiblast-like pluripotent state. This method results in decreased lineage-primed gene expression and marked improvement in directed multilineage differentiation across a broad repertoire of conventional hPSC lines.

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Keywords: Chemical Reversion Conventional Human Pluripotent Stem Cells Naive-like State Multilineage Differentiation LIF-3i Method Pre-implantation Epiblast Lineage-primed Gene Expression Epigenomic Stability Regenerative Medicine HPSC Differentiation Gene Targeting Interspecies Chimeras LIF-5i Medium Cell Dissociation Feeder-free Culture
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