Journal
/
发育生物学
/
Kemiska återgång av konventionella mänskliga pluripotenta stamceller till en naiv-liknande tillstånd med förbättrad Multilinjärt differentiering potens
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
Please note that all translations are automatically generated. Click here for the English version.

Kemiska återgång av konventionella mänskliga pluripotenta stamceller till en naiv-liknande tillstånd med förbättrad Multilinjärt differentiering potens

9,715 Views

•

09:07 min

•

June 10, 2018

DOI:

10.3791/57921-v

DOI:
10.3791/57921-v

•

09:07 min
June 10, 2018

•

1 Views
, , ,
1Department of Oncology, Division of Pediatric Oncology and Institute for Cell Engineering,Johns Hopkins School of Medicine

Tags

Chemical ReversionConventional Human Pluripotent Stem CellsNaive-like StateMultilineage DifferentiationLIF-3i MethodPre-implantation EpiblastLineage-primed Gene ExpressionEpigenomic StabilityRegenerative MedicineHPSC DifferentiationGene TargetingInterspecies ChimerasLIF-5i MediumCell DissociationFeeder-free Culture

Read Article

Cite this Article

Park, T. S., Zimmerlin, L., Evans-Moses, R., Zambidis, E. T. Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency. J. Vis. Exp. (136), e57921, doi:10.3791/57921 (2018).

Download .ris file

Copy

Share Video

.

Copy