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Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

DOI:
10.3791/59833-v

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06:55 min

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July 17, 2019

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, , ,
1Department of Psychiatry and Behavioral Sciences,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine

Chapters

  • 00:04Title
  • 01:232D Culture Differentiation
  • 02:213D Culture Differentiation
  • 03:28Ectoderm Differentiation
  • 04:25Results: Effects of DMSO Pretreatment on Embryonic and Induced Pluripotent Stem Cell Differentiation
  • 06:03Conclusion

Summary

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Generating differentiated cell types from human pluripotent stem cells (hPSCs) holds great therapeutic promise but remains challenging. PSCs often exhibit an inherent inability to differentiate even when stimulated with a proper set of signals. Described here is a simple tool to enhance multilineage differentiation across a variety of PSC lines.

Tags

Pluripotent Stem CellsDMSO TreatmentDifferentiation PrimingCell Replacement TherapyDisease ModelingDrug Screening3D Culture DifferentiationCell Suspension

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