Journal
/
发育生物学
/
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

1,966 Views

•

04:23 min

•

March 31, 2021

DOI:

10.3791/62391-v

DOI:
10.3791/62391-v

•

04:23 min
March 31, 2021

•

6 Views
, , ,
1Department of Cell Biology,University of Virginia, 2Cardiovascular Research Center,University of Virginia, 3Department of Medicine,Yale University School of Medicine, 4Department of Genetics,Yale University School of Medicine, 5Yale Cardiovascular Research Center,Yale University School of Medicine

Summary

Automatically generated

Presented here is a simple protocol for the directed differentiation of hemogenic endothelial cells from human pluripotent stem cells in approximately 1 week.

Explore More Videos

Hemogenic Endothelial CellsHuman Pluripotent Stem CellsDirected DifferentiationFACSCD45CD31VE-cadherinC-KitCD34KDRMethylcelluloseColony Forming UnitBlast Forming UnitHematopoietic Progenitor Cells

Read Article

Cite this Article

Nelson, E. A., Qiu, J., Chavkin, N. W., Hirschi, K. K. Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells. J. Vis. Exp. (169), e62391, doi:10.3791/62391 (2021).

Download .ris file

Copy

Share Video

.

Copy