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在体内荧光亲脂膜染料对大鼠膝骨关节炎模型中人脂肪间充质干细胞的追踪
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In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
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在体内荧光亲脂膜染料对大鼠膝骨关节炎模型中人脂肪间充质干细胞的追踪

8,838 Views

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07:31 min

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October 08, 2017

DOI:

10.3791/56273-v

DOI:
10.3791/56273-v

•

07:31 min
October 08, 2017

•

2 Views
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1Cellular Biomedicine Group, China, 2Cellular Biomedicine Group, California, 3Department of Orthopedics,Shanghai TCM-Integrated Hospital, 4Department of Orthopedics, Shanghai East Hospital,Tongji University

Summary

Automatically generated

该协议描述了一种有效的方法来监测人脂肪来源的骨髓间充质干细胞 (haMSCs) 的细胞持久性和分布的远红色荧光标记的大鼠膝骨关节炎 (KOA) 模型通过关节内 (IA) 注射。

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In Vivo TrackingHuman Adipose-derived Mesenchymal Stem CellsRat Knee Osteoarthritis ModelFluorescent Lipophilic Membrane DyeDiDStem Cell PersistenceBiodistributionRegenerative MedicineAnimal ModelCell LabelingIn Vivo Imaging

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Cite this Article

Li, M., Hao, M., Jiang, D., Chen, Y., Wang, W. In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye. J. Vis. Exp. (128), e56273, doi:10.3791/56273 (2017).

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