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发育生物学
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Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
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发育生物学
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JoVE 杂志 发育生物学
Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

9,899 Views

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11:31 min

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May 30, 2017

DOI:

10.3791/55772-v

DOI:
10.3791/55772-v

•

11:31 min
May 30, 2017

•

5 Views
,
1INRS, Institut Armand-Frappier, 2Biomed, UQAM

Summary

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Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.

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Dianati, E., Plante, I. Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands. J. Vis. Exp. (123), e55772, doi:10.3791/55772 (2017).

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