Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

Elham Dianati; Isabelle Plante

doi:10.3791/55772

/

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

JoVE Journal
発生生物学

このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。

JoVE Journal 発生生物学

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

9,899 Views

•

11:31 min

•

May 30, 2017

DOI:

10.3791/55772-v

DOI:

10.3791/55772-v

•

11:31 min

May 30, 2017

•

5 Views

Elham Dianati^1,2, Isabelle Plante¹

¹INRS, Institut Armand-Frappier, ²Biomed, UQAM

概要

Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.

Explore More Videos

Protein-protein Interactions Co-localization Intercellular Junctions Mammary Glands Co-immunoprecipitation Co-immunofluorescent Staining Formaldehyde Fixation Phosphate-buffered Saline Bovine Serum Albumin Tris-Buffered Saline Polysorbate-20 Primary Antibody Secondary Antibody Fluorescent Conjugation

Read Article

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

概要

Automatically generated

Explore More Videos