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Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
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Chapitres
- 00:05Titre
- 00:48Identifying Proetin Co-localization
- 03:41Studying Protein-protein Interactions (PPIs): Indirect Immunoprecipitation
- 06:29Downstream Application: Gel Electrophoresis Followed by Western Blot
- 08:26Results: Interaction of Gap Junction, Adhered Junction, and Tight Junction Components in the Murine Mammary Gland
- 10:21Conclusion
Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.
