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Biologia do Desenvolvimento
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Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

DOI:
10.3791/55772-v

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11:31 min

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May 30, 2017

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,
1INRS, Institut Armand-Frappier, 2Biomed, UQAM

Capítulos

  • 00:05Título
  • 00:48Identifying Proetin Co-localization
  • 03:41Studying Protein-protein Interactions (PPIs): Indirect Immunoprecipitation
  • 06:29Downstream Application: Gel Electrophoresis Followed by Western Blot
  • 08:26Results: Interaction of Gap Junction, Adhered Junction, and Tight Junction Components in the Murine Mammary Gland
  • 10:21Conclusion

Summary

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Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.

Tags

Keywords: Protein-protein InteractionsCo-localizationIntercellular JunctionsMammary GlandsCo-immunoprecipitationCo-immunofluorescent StainingFormaldehyde FixationPhosphate-buffered SalineBovine Serum AlbuminTris-Buffered SalinePolysorbate-20Primary AntibodySecondary AntibodyFluorescent Conjugation

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