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Journal / Biochemistry / 时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态…
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JoVE Journal Biochemistry
时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态
 
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时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态

Article DOI: 10.3791/55464-v • 09:18 min • April 17th, 2017
April 17th, 2017 •
1, 1, 1, 1, 1, 1,2,3
1Department of Chemistry, York University, 2The Centre for Research in Mass Spectrometry, York University, 3The Centre for Research on Biomolecular Interactions, York University

章节

总结概括
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Click here for the English version.

构象的灵活性在蛋白质功能的关键作用。在此，我们描述了使用耦合到氢 - 氘交换用于探测驱动功能在有序和无序的蛋白质的快速结构变化的时间分辨电喷雾电离质谱法。

Tags

生物化学，第122，时间分辨电喷雾电离，质谱，氢 - 氘交换，蛋白质动力学，本质无序蛋白，微流体
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