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Resuelta en el tiempo de ionización por electrospray de hidrógeno-deuterio Espectrometría de Masas de cambio para el estudio de la estructura proteica y Dinámica
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Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
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Resuelta en el tiempo de ionización por electrospray de hidrógeno-deuterio Espectrometría de Masas de cambio para el estudio de la estructura proteica y Dinámica

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09:18 min

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April 17, 2017

DOI:

10.3791/55464-v

DOI:
10.3791/55464-v

•

09:18 min
April 17, 2017

•

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, , , , ,
1Department of Chemistry,York University, 2The Centre for Research in Mass Spectrometry,York University, 3The Centre for Research on Biomolecular Interactions,York University

Tags

Time-resolved Electrospray IonizationHydrogen-deuterium ExchangeMass SpectrometryProtein StructureProtein DynamicsConformational FlexibilityTransient Protein ConformationsLoop RegionsMolten GlobulesIntrinsically Disordered ProteinsNeurodegenerative DisordersProtein MisfoldingProtein AggregationEnzyme Catalytic TurnoverProtein-protein InteractionsProtein-ligand InteractionsContinuous FlowTime-resolved Kinetic MixerOrthogonal Mixing

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Lento, C., Zhu, S., Brown, K. A., Knox, R., Liuni, P., Wilson, D. J. Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics. J. Vis. Exp. (122), e55464, doi:10.3791/55464 (2017).

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