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Time-løst elektrosprayioniserings Brint-deuterium Exchange-massespektrometri til at studere proteiners struktur og Dynamics

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生物化学

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JoVE 杂志生物化学

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Time-løst elektrosprayioniserings Brint-deuterium Exchange-massespektrometri til at studere proteiners struktur og Dynamics

9,599 Views

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09:18 min

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April 17, 2017

DOI:

10.3791/55464-v

DOI:

10.3791/55464-v

•

09:18 min

April 17, 2017

•

1 Views

Cristina Lento, Shaolong Zhu, Kerene A. Brown, Ruth Knox, Peter Liuni, Derek J. Wilson^2,3

¹Department of Chemistry,York University, ²The Centre for Research in Mass Spectrometry,York University, ³The Centre for Research on Biomolecular Interactions,York University

Tags

Time-resolved Electrospray Ionization Hydrogen-deuterium Exchange Mass Spectrometry Protein Structure Protein Dynamics Conformational Flexibility Transient Protein Conformations Loop Regions Molten Globules Intrinsically Disordered Proteins Neurodegenerative Disorders Protein Misfolding Protein Aggregation Enzyme Catalytic Turnover Protein-protein Interactions Protein-ligand Interactions Continuous Flow Time-resolved Kinetic Mixer Orthogonal Mixing

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