/

/

毛细管电泳电喷雾电离质谱法绝对定量肌醇焦磷酸盐

JoVE 杂志
生物化学

This content is Free Access.

JoVE 杂志生物化学

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Please note that all translations are automatically generated. Click here for the English version.

毛细管电泳电喷雾电离质谱法绝对定量肌醇焦磷酸盐

DOI:

10.3791/62847-v

•

09:22 min

•

August 13, 2021

•

Danye Qiu, Verena B. Eisenbeis, Adolfo Saiardi, Henning J. Jessen

¹Institute of Organic Chemistry,University of Freiburg, ²Medical Research Council, Laboratory for Molecular Cell Biology,University College London

Chapters

00:04Introduction
00:45Setting Up the Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS) System
01:38Preparation of Buffer, Capillary, and CE-MS System
03:28Performing the CE-ESI-MS Runs
05:10Data Analysis
06:50Results: Quantitation of Inositol Pyrophosphates from Mammalian Cell Extracts by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
08:35Conclusion

Summary

自动翻译

描述了毛细管电泳电喷雾电离质谱法用于绝对定量哺乳动物细胞提取物中肌醇焦磷酸盐的程序。

Tags

Inositol Pyrophosphates Capillary Electrophoresis Electrospray Ionization Mass Spectrometry CE-ESI-MS Inositol Phosphate Metabolism Quantitation Biosynthetic Pathway CE Running Buffer Sheath Liquid Capillary Column ESI Sprayer Inositol Phosphate Standards

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

鞘毛细管电泳 - 质谱联用生物样品的代谢谱

使用中性毛细管单克隆抗体亚型的毛细管电泳分离

时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态

解决由蓝变性PAGE和蛋白质相关剖析亲和纯化的蛋白复合物

多肽基基质辅助激光解吸/电离质谱成像中福尔马林固定样品的优化制备工作流

毛细管电泳与亲和毛细管电泳对金属离子 AtHIRD11 固有紊乱与结合的分析

叶喷雾质谱: 一种快速的环境电离技术直接评价植物组织中的代谢产物

血管紧张蛋白II图解的硝酸分析与鉴定

通过亲和色谱与西布洛特或质谱结合的亲致色谱,识别磷酸磷酸磷酸磷酸醇或磷酸蛋白

探头电喷电镀质谱的样品制备

Read Article