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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
 

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

Article DOI: 10.3791/52121-v • 10:39 min • November 27th, 2014
November 27th, 2014 •
1, 2, 2, 1,2,3, 1,2,3
1Department of Microbiology & Immunology, The University of Texas Medical Branch, 2Department of Pathology, The University of Texas Medical Branch, 3Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development, The University of Texas Medical Branch

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Automatic Translation

Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in mice.

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In Vitro Analysis Myd88-mediated Cellular Immune Response West Nile Virus Mutant Strain Infection Attenuated West Nile Virus Nonstructural (NS) 4B-P38G Mutant Innate Cytokine Response T Cell Response Wild-type WNV Myeloid Differentiation Factor 88 (MyD88) Signaling T Cell Priming Assay Intracellular Cytokine Staining (ICS) Dendritic Cells (DCs) T Cell Functions Cell Proliferation Flow Cytometry Carboxyfluorescein Succinimidyl Ester (CFSE) Labeled CD4+ T Cells OTII Transgenic Mice Radioactive Reagents Microcentrifuge Tube System Cell Culture Cytokine Staining Procedures ICS Protocol
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