需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques
20,897 Views
•
07:11 min
•
April 22, 2010
DOI:
Summary
Automatically generated
We demonstrate the utility of multicolor flow cytometry for detailed phenotypic and functional characterization of total as well as memory subsets of CD4+ and CD8+ T cells in rhesus macaques, the ideal model for HIV/AIDS vaccine studies.
Explore More Videos
Multicolor Flow CytometryCellular Immune ResponseRhesus MacaquesHIV-AIDS ModelPathogenesisVaccinesTherapeuticsPhenotypic AnalysisFunctional AnalysisIntracellular Cytokine ProductionCD4+ T CellsCD8+ T CellsMemory SubsetsInterferon Gamma (INF-)Interleukin (IL)-2PMA Plus Ionomycin (PMA+I)Cytokine ProfilesMemory Cells SubsetsAntigen-specific CD4+ T CellsAntigen-specific CD8+ T CellsPBMC SamplesHIV Envelope Peptide Cocktail Vaccine
Read Article
Cite this Article
He, H., Courtney, A. N., Wieder, E., Sastry, K. J. Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques. J. Vis. Exp. (38), e1743, doi:10.3791/1743 (2010).
Copy