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免疫与感染
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Hurtig screening af HIV revers transkriptase og Integrase hæmmere
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Hurtig screening af HIV revers transkriptase og Integrase hæmmere

DOI:
10.3791/51400-v

•

05:46 min

•

April 09, 2014

•

,
1HIV Drug Resistance Program,National Cancer Institute

Chapters

  • 00:05Title
  • 01:09Viral Stock Generation
  • 02:29Compound Screening
  • 03:42Measurement of Luciferase Activity
  • 04:41Results: Representative Luciferase Signal Readout Inhibition
  • 05:22Conclusion

Summary

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Her beskriver vi cellulær cytotoksicitet og enkelt runde infektivitet analyser, som giver mulighed for hurtig og præcis screening af forbindelser for at bestemme deres cellulære cytotoksicitet (CC 50) og IC50-værdier mod WT og resistent HIV-1 lægemiddel.

Tags

HIV Reverse TranscriptaseHIV IntegraseAntiviral CompoundsDrug ResistanceCytotoxicity AssayLuciferase ReporterViral InfectivityScreening PlatformHigh-throughput Screening

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