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Immunology and Infection

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JoVE Journal Immunology and Infection
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
 

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

Article DOI: 10.3791/50606-v • 16:04 min • June 19th, 2013
June 19th, 2013 •
1, 1, 1, 1
1Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles

章节

总结概括
Automatic Translation

Here, we use retroviral transduction and concatemeric transfection to create a cell line that can express the components of a lentiviral vector (LV) in the absence of tetracycline. This LV encodes GFP and is pseudotyped with a glycoprotein, SVGmu, which is specific for a receptor on dendritic cells.

Tags

Keywords: Lentiviral Vectors High-titer Dendritic Cells Tet-off Producer Cell Line Concatemeric DNA Transfection Reporter Gene Transduction T Cell Immune Response
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