需要订阅 JoVE 才能查看此. 登录或开始免费试用。
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
14,399 Views
•
16:04 min
•
June 19, 2013
DOI:
Summary
Automatically generated
Here, we use retroviral transduction and concatemeric transfection to create a cell line that can express the components of a lentiviral vector (LV) in the absence of tetracycline. This LV encodes GFP and is pseudotyped with a glycoprotein, SVGmu, which is specific for a receptor on dendritic cells.
Read Article
Cite this Article
Bryson, P. D., Zhang, C., Lee, C., Wang, P. A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells. J. Vis. Exp. (76), e50606, doi:10.3791/50606 (2013).
Copy