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Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
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July 08, 2011
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We present our optimized high-throughput nucleofection protocol as an efficient way of transfecting primary human monocyte-derived dendritic cells with either plasmid DNA or siRNA without causing cell maturation. We further provide evidence for successful siRNA silencing of targeted gene RIG-I at both the mRNA and protein levels.
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Dendritic CellsImmune SystemInfectionPathogen-associated Molecular Patterns (PAMPs)Pattern Recognition Receptors (PRRs)Cell MaturationType 1 InterferonProinflammatory CytokinesMHCIICD86Adaptive Immune ResponseMolecular NetworksSignaling PathwaysInduced GenesDC-based VaccinesTransfection Of Dendritic Cells
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Bowles, R., Patil, S., Pincas, H., Sealfon, S. C. Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation. J. Vis. Exp. (53), e2766, doi:10.3791/2766 (2011).
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