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Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
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11:18 min
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May 07, 2012
DOI:
1Center for Cell and Gene Therapy,Baylor College of Medicine , 2Pathology and Immunology,Baylor College of Medicine , 3Department of Stem Cell Transplantation and Cellular Therapy,University of Texas M.D. Anderson Cancer Center, 4医学,Baylor College of Medicine , 5Department of Pediatrics,Baylor College of Medicine
Summary
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Here we describe the first good manufacturing practice (GMP)-compliant method of producing virus-specific cytotoxic T lymphocytes (CTL) from umbilical cord blood, a source of predominantly naîve T cells.
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Hanley, P. J., Lam, S., Shpall, E. J., Bollard, C. M. Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus. J. Vis. Exp. (63), e3627, doi:10.3791/3627 (2012).
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