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Journal / Neuroscience / Targeting Olfactory Bulb Neurons Using Combined…
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Neuroscience

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JoVE Journal Neuroscience
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
 

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

Article DOI: 10.3791/2964-v • 08:18 min • August 15th, 2011
August 15th, 2011 •
1, 2, 3, 1
1Department of Biology, Pace University, 2Cellular and Molecular Medicine, University of California, San Diego, 3Division of Cell Biology and Cell Physiology, Zoological Institute, Braunschweig University of Technology

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The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.

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Olfactory Bulb Neurons In Vivo Electroporation Gal4-based Enhancer Trap Zebrafish Lines DNA Expression Plasmids RNAi Reagents Morpholino Anti-sense Oligonucleotides Developing Embryos C. Elegans Chick Xenopus Mouse Spatial Resolution Temporal Resolution Transgenic Approaches Specific Cell Population Developing Nervous System
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