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Journal / Neuroscience / Mapping and Application of Enhancer-trap Flippase …
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Neuroscience

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JoVE Journal Neuroscience
Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
 

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Article DOI: 10.3791/2649-v • 09:45 min • June 3rd, 2011
June 3rd, 2011 •
1, 1, 1, 1, 1,2, 1, 1, 1, 1
1Department of Zoology, University of Oklahoma - Norman, 2Department of Biology, Brandeis University

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We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.

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Gal4/UAS Binary Method Gene Manipulation Neural Circuitry Manipulation FINGR Method Gal4 Expression Tissue-specific Expression Gal80 Converting Tools Enhancer-trap FLP UAS-effector UAS-reporter Morphological Analysis TubP>Gal80> ('flip Out') TubP>stop>Gal80 ('flip In') Tissue-specific ET-FLP
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