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Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

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09:45 min

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June 03, 2011

DOI:

10.3791/2649-v

DOI:
10.3791/2649-v

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09:45 min
June 03, 2011

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3 Views
, , , , , , , ,
1Department of Zoology,University of Oklahoma – Norman, 2Department of Biology,Brandeis University

Summary

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We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.

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Gal4/UAS Binary MethodGene ManipulationNeural Circuitry ManipulationFINGR MethodGal4 ExpressionGal80 Converting ToolsEnhancer-trap FLPUAS-effectorUAS-reporterMorphological AnalysisTubP>Gal80> (‘flip Out’)TubP>stop>Gal80 (‘flip In’)

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Fore, T. R., Ojwang, A. A., Warner, M. L., Peng, X., Bohm, R. A., Welch, W. P., Goodnight, L. K., Bao, H., Zhang, B. Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS. J. Vis. Exp. (52), e2649, doi:10.3791/2649 (2011).

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