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मास स्पेक्ट्रोमेट्री के साथ युग्मित पेप्टाइड Immunoaffinity संवर्धन का उपयोग प्रोटीन की मात्रा का ठहराव
JoVE 杂志
生物学
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JoVE 杂志 生物学
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
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मास स्पेक्ट्रोमेट्री के साथ युग्मित पेप्टाइड Immunoaffinity संवर्धन का उपयोग प्रोटीन की मात्रा का ठहराव

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06:09 min

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July 31, 2011

DOI:

10.3791/2812-v

DOI:
10.3791/2812-v

•

06:09 min
July 31, 2011

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9 Views
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1Clinical Research Division,Fred Hutchinson Cancer Research Center – FHCRC, 2Department of Biochemistry and Microbiology,University of Victoria, 3Broad Institute of MIT and Harvard, 4Genome BC Proteomics Centre,University of Victoria, 5Plasma Proteome Institute

Tags

QuantificationProteinsPeptide Immunoaffinity EnrichmentMass SpectrometryTraditional ImmunoassaysSISCAPAStable Isotope StandardsCapture By Anti-peptide AntibodiesSRM/MRM-MSSmall MoleculesProtein ConcentrationProteotypic PeptidesTrypsin

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Zhao, L., Whiteaker, J. R., Pope, M. E., Kuhn, E., Jackson, A., Anderson, N. L., Pearson, T. W., Carr, S. A., Paulovich, A. G. Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry. J. Vis. Exp. (53), e2812, doi:10.3791/2812 (2011).

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