需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
26,526 Views
•
13:21 min
•
November 20, 2010
DOI:
Explore More Videos
IsolationExpansionAdult MouseNeural Stem CellsNeurosphere AssayPutativeMurine BrainReynolds And WeissChemically Defined Serum-free Culture SystemGrowth Factor Responsive Precursor CellsEpidermal Growth FactorBasic Fibroblastic Growth FactorColoniesUndifferentiated CellsNeurospheresSubculturedPool Of Neural Stem CellsDifferentiateThree Major Cell TypesCNSNeuronsAstrocytesOligodendrocytesInvaluable ToolIn Vitro StudiesTherapeutic PurposesVideo DemonstrationPeriventricular RegionReproducible Neurosphere Cultures
Read Article
Cite this Article
Azari, H., Rahman, M., Sharififar, S., Reynolds, B. A. Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay. J. Vis. Exp. (45), e2393, doi:10.3791/2393 (2010).
Copy