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La generación de células iPS a partir de MEFs través de la expresión forzada de Sox-2, Oct-4, c-Myc y Klf4
JoVE Journal
Biology
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JoVE Journal Biology
Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
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La generación de células iPS a partir de MEFs través de la expresión forzada de Sox-2, Oct-4, c-Myc y Klf4

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35,798 Views

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13:02 min

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April 07, 2008

DOI:

10.3791/734-v

DOI:
10.3791/734-v

•

13:02 min
April 07, 2008

•

13 Views
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1Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

Summary

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Este video muestra el procedimiento para generar células madre pluripotentes inducidas mediante lentivirus inducible que expresan Oct4, Sox2, c-Myc y Klf4.

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IPS CellsMEFSForced ExpressionSox-2Oct-4C-MycKlf4PluripotencyViral TransductionReprogramming StrategyDoxycycline-inducible Lentiviral VectorsInduced Pluripotent Stem CellsMouse Embryonic FibroblastsInducible LentivirusesRetroviral InfectionGene Expression Analysis

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Cite this Article

Welstead, G. G., Brambrink, T., Jaenisch, R. Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4. J. Vis. Exp. (14), e734, doi:10.3791/734 (2008).

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