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Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

JoVE Journal
Biologie

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JoVE Journal Biologie

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

35,800 Views

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13:02 min

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April 07, 2008

DOI:

DOI:

•

13:02 min

April 07, 2008

•

13 Views

G. Grant Welstead¹, Tobias Brambrink¹, Rudolf Jaenisch¹

¹Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

Summary

This video shows the procedure for generating induced pluripotent stem cells using inducible lentivirus that express Oct4, Sox2, c-Myc and Klf4.

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IPS Cells MEFS Forced Expression Sox-2 Oct-4 C-Myc Klf4 Pluripotency Viral Transduction Reprogramming Strategy Doxycycline-inducible Lentiviral Vectors Induced Pluripotent Stem Cells Mouse Embryonic Fibroblasts Inducible Lentiviruses Retroviral Infection Gene Expression Analysis

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