Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

G. Grant Welstead; Tobias Brambrink; Rudolf Jaenisch

doi:10.3791/734

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Geração de iPS a partir de células MEFS através da expressão forçada de Sox-2, Oct-4, c-Myc e KLF4

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Biology

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JoVE Journal Biology

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Geração de iPS a partir de células MEFS através da expressão forçada de Sox-2, Oct-4, c-Myc e KLF4

35,800 Views

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13:02 min

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April 07, 2008

DOI:

10.3791/734-v

DOI:

10.3791/734-v

•

13:02 min

April 07, 2008

•

13 Views

G. Grant Welstead¹, Tobias Brambrink¹, Rudolf Jaenisch¹

¹Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

Summary

Este vídeo mostra o procedimento para gerar células-tronco pluripotentes induzidas usando lentivírus induzível que expressam Oct4, Sox2, c-Myc e KLF4.

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IPS Cells MEFS Forced Expression Sox-2 Oct-4 C-Myc Klf4 Pluripotency Viral Transduction Reprogramming Strategy Doxycycline-inducible Lentiviral Vectors Induced Pluripotent Stem Cells Mouse Embryonic Fibroblasts Inducible Lentiviruses Retroviral Infection Gene Expression Analysis

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Geração de iPS a partir de células MEFS através da expressão forçada de Sox-2, Oct-4, c-Myc e KLF4

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Summary

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