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Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation
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Developmental Biology
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JoVE Journal Developmental Biology
Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

DOI:
10.3791/66682-v

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07:30 min

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June 28, 2024

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, , , , ,
1Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine,Bond University

Chapters

  • 00:02Introduction
  • 00:58Matrix Encapsulation of Cell Aggregates
  • 02:52Three-Dimensional Organoid Culture
  • 03:16Kidney Capsule Transplantation
  • 04:54Results: Preparation and Application of Matrix-Encapsulated Spleen Stromal Cell Aggregates
  • 06:27Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a protocol for aggregating and encapsulating spleen cells within a semi-solid basement membrane matrix. Basement membrane matrix constructs can be used in three-dimensional culture for studying organoid development, or for in vivo transplantation and tissue regeneration studies.

Tags

Cell AggregationBasement Membrane MatrixMatrigelCell CultureIn Vivo TransplantationCell PelletCentrifugation

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