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Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation
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Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation
Chapitres
- 00:02Introduction
- 00:58Matrix Encapsulation of Cell Aggregates
- 02:52Three-Dimensional Organoid Culture
- 03:16Kidney Capsule Transplantation
- 04:54Results: Preparation and Application of Matrix-Encapsulated Spleen Stromal Cell Aggregates
- 06:27Conclusion
This article describes a protocol for aggregating and encapsulating spleen cells within a semi-solid basement membrane matrix. Basement membrane matrix constructs can be used in three-dimensional culture for studying organoid development, or for in vivo transplantation and tissue regeneration studies.
