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Inmunofluorescencia dual de γH2AX y 53BP1 en linfocitos periféricos humanos
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Genetics
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JoVE Journal Genetics
Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
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Inmunofluorescencia dual de γH2AX y 53BP1 en linfocitos periféricos humanos

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1,058 Views

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05:34 min

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July 14, 2023

DOI:

10.3791/65472-v

DOI:
10.3791/65472-v

•

05:34 min
July 14, 2023

•

7 Views
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1Unità di Genetica, Dipartimento di Biologia,University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology,Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico

Summary

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Este protocolo presenta un método para evaluar la formación y reparación de roturas de doble cadena de ADN mediante la detección simultánea de focos γH2AX y 53BP1 en núcleos interfase de linfocitos periféricos humanos tratados con bleomicina.

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Cite this Article

Falaschi, A., Chiaramonte, A., Testi, S., Scarpato, R. Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes. J. Vis. Exp. (197), e65472, doi:10.3791/65472 (2023).

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