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Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
JoVE Journal
Genética
Author Produced
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JoVE Journal Genética
Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

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05:34 min

July 14, 2023

DOI:

05:34 min
July 14, 2023

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Summary

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This protocol presents a method to assess the formation and repair of DNA double-strand breaks through the simultaneous detection of γH2AX and 53BP1 foci in interphase nuclei of bleomycin-treated human peripheral lymphocytes.

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